SULFO NHS LC BIOTIN

Spacer arm 22.4 angstroms. Biotinylation reagent. Readily couples biotin to primary amino groups under mild conditions. Labels antibodies, hormones or other polypeptide molecules containing lysine residues. Purity >94% by HPLC.

Arosa, F.A., et al, J. Biol. Chem., 274, 16917-16922 (1999). 
Schuberth, H.J., et al, J. Immunol. Methods, 189, 89-98 (1996). 
Baqui, M., et al, J. Biol. Chem., 278, 1206-1211 (2003). 
Huh, K.H., Wenthold, R.J., J. Biol. Chem., 274, 151-157 (1999). 
Foxall, C., , et al, Anal. Biochem., 231, 366-373 (1995). 
Hnatowich, D.J., et al, J. Nucl. Med., 28, 1294-1302 (1987). 
Kerstens, H.M., et al, J. Histochem. Cytochem., 43(4), 347-352 (1995). 
Corbeil, D., et al, J. Biol. Chem., 275, 5512-5520 (2000). 
Lesa, G.M., et al, J. Biol. Chem., 275, 2831-2836 (2000). 
Liaw., P.C.Y., et al, J. Biol. Chem., 276, 8364-8370 (2001). 
Lui, L.A., Engvall, E., J. Biol. Chem., 274, 38171-38176 (1999).

Sulfo NHS LC biotin biotinylates, amino acids, peptides or proteins under mild conditions 
Sulfo NHS LC biotin biotinylates only surface proteins of whole cells because the negatively charged reagent does not permeate cell membranes 
Soluble in water. The charge of the sulfo NHS sodium salt increases the water solubility 
NHS biotin is used to biotinylate antibodies or other preins for detection or purification using streptavidin probes or resin 
Forms stable amide bonds therefore the reaction is not reversable. The spacer arm cannot be cleaved.