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(Biotinylation Reagent)

B108   Biotin hydrazide
[ 66640-86-6 ]   f.w.: 258.3 daltons

Hydrazide biotin is a short length, hydrazide activated biotinylation reagent for labeling glycoproteins and other carbohydrate containing compounds having oxidizable sugars or aldehydes.

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100mgm + $113.00
250mgm + $267.00
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Spacer arm 15.7 angstroms. Carbohydrate reactive biotinylation reagent. Biotinylates immunoglobulins without loss of immunological activity. Use at pH 4-6 to complete coupling reaction. Purity >95% by TLC.

  1. O\'Shannessey, D.J., et al. (1984) A novel procedure for labeling immunoglobulins by conjugation to oligosaccharide moieties. Immunol. Lett. 8, 273-277. 
  2. Kähne, T. and Ansorge, S. (1994) Non-radioactive labeling and immunoprecipitation analysis of leukocyte surface proteins using different methods of protein biotinylation. J. Immunol. Methods 168, 209-218. 
  3. Bayer, E.A., et al. (1988) Biocytin hydrazide-a selective label for sialic acids, galactose, and other sugars in glycoconjugates using avidin-biotin technology. Anal. Biochem. 170, 271-281.O’Shannessey, D.J. and Quarles, R.H. (1987) Labeling of the oligosaccharide moieties of immunoglobulins. J. Immunol. Meth. 99, 153-161. 
  4. O\'Shannessy, D. J. and Quarles R.H. (1985) Specific conjugation reactions of the oligosaccharide moieties of immunoglobulins. J. Appl. Biochem. 7, 347-355. 
  5. O\'Shannessey, D.J., et al. (1987) Quantitation of glycoproteins on electroblots using the biotin-streptavidin complex. Anal. Biochem. 163, 204-209. 
  6. Reisfield, A., et al. (1987) Nonradioactive hybridization probes prepared by the reaction of biotin hydrazide with DNA. Biochem. Biophys. Res. Com. 142, 519-526. 
  7. Rosenberg , M.B., et al. (1986) Receptor binding activities of biotinylated derivatives of ß-nerve growth factor. J. Neurochem. 46, 641-648. 
  8. Scott, M.G., et al. (2000) Cationic Antimicrobial Peptides Block the Binding of Lipopolysaccharide (LPS) to LPS Binding Protein. J. Immunol. 164, 549-553. 
  9. Wade, D.P., et al. (1985) Detection of the low density-lipoprotein receptor with biotin-low density lipoprotein. Biochem. J. 229, 785-790.
  • Biotin hydrazide biotinylates glycosylated proteins at sialic acid residues for detection or purification using streptavidin probes or resins 
  • Biotinylate and isolate cell surface glycoproteins
  • Aldehyde reactive reacts with aldehydes formed by periodate oxidation of sugar groups 
  • Hydrazide activated perform reactions at pH 4 to 6 in buffers such as sodium acetate 
  • Biotin hydrazide is irreversible and forms semi-permanent hydrazone bonds; spacer arm cannot be cleaved 
  • Solubility-usually dissolved in DMSO before further dilution in aqueous buffers 
  • Spacer arm 15.7 angstroms