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(Protein Crosslinker heterobifunctional)

CL222   N-(epsilon-Maleimidocaproyloxy)succinimide
[ 55750-63-5 ]   f.w.: 308.3 daltons

EMCS is an amine-to-sulfhydryl crosslinker that contains NHS ester and maleimide reactive groups at opposite ends of a medium length spacer arm (9.4 angstroms).

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50mgm + $196.00
100mgm + $370.00
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Spacer arm 9.4 angstroms. Heterobifunctional crosslinker. Sulfhydryl and amine reactive reagent used for enzyme immunoassay. Non-cleavable, water insoluble. Aliphatic spacer offers low potential for eliciting an immune response.

  1. Fujiwara, K., et al. (1988). J. Immunol. Methods 112, 77-83. 
  2. Peeters, J.M., et al. (1989). J. Immunol. Methods 120, 133-143. 
  3. Hermanson, G.T. (1996). Bioconjugate Techniques, San Diego: Academic Press, pp. 243-245.
  • NHS ester end couples with primary amines at pH 7-9 to form stable amide bonds
  • Maleimides react with -SH groups at pH 6.5-7.5, forming stable thioether linkages
  • Non-cleavable, water-insoluble
  • Increased sphere of coupling vs. GMBS
  • Aliphatic spacer offers low potential for eliciting an immune response
  • Reactive groups: maleimide and NHS ester
  • Reactive toward: sulfhydryl and amino groups