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(Protein Crosslinker homobifunctional)

CL100   Disuccinimidylsuberate
[ 68528-80-3 ]   f.w.: 368.3 daltons

DSS is a non cleavable and membrane permeable crosslinker that contains an amine reactive N-hydroxysuccinimide (NHS) ester at each end of an 8-carbon spacer arm.

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2gm + $212.00
5gm + $529.00
10gm + $1000.00
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Spacer arm 11.4 angstroms. Homobifunctional reagent. Noncleaveable amine reactive homobifunctional crosslinker. Has been used for conjugating radiolabeled ligand to cell surface receptor. Purity >95% by HPLC.

  1. Chataway, T.K. and Barritt, G.J. (1995). Mol. Cell. Biochem. 145(2), 111-120. 
  2. Donato, R., et al. (1989). J. Neurochem. 53(2), 566-571. 
  3. Ishmael, F.T., et al. (2005) Intersubunit and Domain Interactions of the Meprin B Metalloproteinase: Disulfide Bonds and Protein-Protein Interactions in the MAM and TRAF Domains. J. Biol. Chem.280, 13895-13901. 
  4. Longshaw, V.M., et al. (2004) Nuclear translocation of the Hsp70/Hsp90 organizing protein mSTI1 is regulated by cell cycle kinases. J. Cell Sci. 117, 701-710. 
  5. Mattson, G., et al. (1993). A practical approach to crosslinking. Molecular Biology Reports 17, 167-183. 
  6. Mathieu, M., et al. (1990). Mol. Endocrinol. 4(9), 1327-1335. 
  7. Partis, M.D., et al. (1983). Crosslinking of proteins by omega-maleimido alkanoyl N-hydroxysuccinimide esters. J. Protein. Chem. 2, 263-277.
  • Amine reactive Sulfo NHS ester reacts rapidly with any primary amine containing molecule
  • DSS crosslinker is membrane permeable, so intracellular crosslinking can be done
  • High purity, crystalline DSS can be used to create high-purity conjugates