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(Biochemical Reagent)

BR100   5-Bromo-4-chloro-3-phosphate-p-toluidine salt
[ 6578-6-9 ]   f.w.: 433.6 daltons

BCIP-T (5-bromo-4-chloro-3-indolyl phosphate, p-toluidine salt) is a chromogenic substrate for alkaline phosphatase used in conjunction with the oxidant NBT (nitro blue tetrazolium) to enhance blue color development. Alkaline phosphatase catalyses the removal of a phosphate group from BCIP-T, generating a product that oxidizes and dimerizes to dibromodichloro indigo. The reducing equivalents produced during the dimerization reaction reduce NBT to an insoluble purple dye, diformazan.

Quantity Price  
1gm $95.00
5gm + $470.00
500mgm + $59.00

Preparation of a 50 mg/mL stock solution in dimethylformamide
1.J. Sambrook, D. W. Russell, Molecular Cloning: A Laboratory Manual, the Third edition (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 16.42, 2001).