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PITC Edman's Reagent

(Electrophoresis Reagent)

E110   Phenylisothiocyanate
[ 103-72-0 ]   f.w.: 135.2 daltons

PITC, phenylisothiocyanate, also known as Edman's Reagent, enables the sequential degradation of amino acids in a polypeptide chain, yielding primary structural information. PITC reacts readily with amino acids at alkaline pH. Precolumn derivatization results in phenylthiocarbamyl derivatives (PTC-amino acids) that can be separated and quantified using reverse-phase HPLC. This method produces stable products with all amino acids, including proline. PITC is volatile, making it possible to remove excess reagent in vacuo, thereby minimizing the possibility of reagent interference. Detection of picomole quantities of the derivatives can be achieved using a UV detector at 254nm. PITC derivatization followed by reverse-phase chromatography can be used for identification and quantitation of methylated, halogenated, phosphorylated and sulfonated amino acids.

Quantity Price  
10gm $34.20
25gm $80.75

1.Edman, P. (1950). Preparation of phenylthiohydrantoins from natural amino acids. Acta Chem Scand 4:277-82. 2.Edman, P. and Begg, G. (1967). A protein seqenator. Eur J Biochem 1(1):80-91. 3.Heinrikson, R.L. and Meridith, S.C. (1984). Amino acid analysis by reverse-phase high-performance liquid chromatography: Precolumn derivatization with phenylisothiocyanate. Anal Biochem 136:65-74. 4.Schoze, H. (1985). Determination of phenylthiocarbamyl amino acids by reverse-phase high-performance liquid chromatography. J Chromatogr 350:453-60. 5.Ebert, R.F. (1986). Amino acid analysis by HPLC: Optimized conditions for chromatography of phenylthiocarbamyl derivatives. Anal Biochem 154:431-5. 6.Cohen, S.A. and Strydom, D.J. (1988). Amino acid analysis utilizing phenyliosthiocyanate derivatives. Anal Biochem 174:1-16.

  • For pre-column derivatization of amino acids for picomole detection levels in HPLC
  • Reacts with amine groups of amino acids in 5 to 10 minutes at room temperature to yield products that are detectable by UV absorbance (Emax = 254nm)
  • Produces phenylthiocarbamyl (PTC) derivatives that can be separated and quantified in 30 minutes using reverse-phase HPLC
  • Produces stable products with all amino acids, including proline