Home > Products > NHS LC BIOTIN

NHS LC BIOTIN

(Biotinylation Reagent)


B104   Succinimidyl-6-(biotinamido)hexanoate
[ 72040-63-2 ]   f.w.: 455.5 daltons

NHS LC Biotin has an extended spacer arm and reduces steric hindrance when binding several biotinylated molecules to one avidin complex.



Quantity Price  
50mgm $81.00
100mgm $153.00
Download***Certificate of Analysis***


Spacer arm 22.4 angstroms. Biotinylation reagent. Reacts with primary amines to biotinylate proteins under mild conditions. Purity >94% by TLC. Soluble in DMSO.

  1. Burgess, T.L., et al, J. Biol. Chem., 270(32), 19188-19196 (1995). 
  2. Gretch, D.R., et al, Anal. Biochem., 163, 270-277 (1987). 
  3. Ying, S.C., et al, J. Immunol., 143(1), 221-228 (1989). 
  4. Herwald, H., et al, J. Biol. Chem., 270(24), 14634-14642 (1995) 
  5. Jans, D.A.., , et al, J. Biol. Chem., 265(24), 14599-14605 (1990). 
  6. Schumacher, T.N., et al, Science, 271, 1854-1857 (1996). 
  7. Tang, A., et al, Nature, 361, 82-85 (1993). 
  8. Chui, N.H, et al, Clin. Chem., 45, 1954-1959 (1999).
  • NHS LC biotin biotinylates, amino acids, peptides or proteins under mild conditions
  • Penetrates cell membranes and can be used to label inside cells
  • Soluble in DMSO and DMF. Dissolve in DMSO or DMF prior to dilution in aqueous systems
  • NHS LC biotin is used to biotinylate antibodies or other proteins for detection or purification using streptavidin probes or resin
  • Forms stable amide bonds therefore the reaction is not reversable