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BIOTIN BMCC

(Biotinylation Reagent)


B111   (1-Biotinamido)-4[4-(maleimidomethyl)cyclohexanecarboxamido]hexane
  f.w.: 533.7 daltons

Biotin BMCC is a maleimide activated, sulfhydryl reactive biotinylation reagent with an extended spacer arm that contains a stabilizing cyclohexane group.



Quantity Price  
50mgm $126.00
100mgm $238.00
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Spacer arm 32.6 angstroms. Biotinylation reagent. Biotinylates at acidic or neutral pH. The reaction avoids potential modification of tyrosine groups that can inactivate biological activity. Soluble in DMSO and DMF.

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Protein labeling biotinylate antibodies or other proteins for use in protein methods

Membrane permeable Biotin BMCC can be used to label inside cells (intracellular)

Reacts with sulfhydryls (-SH), such as the side-chain of cysteine

Maleimide activated reactions at pH 6.5 to 7.5 in buffers such as PBS

Biotin BMCC is irreversible It forms permanent thioether bonds; spacer arm cannot be cleaved

Biotin BMCC must be dissolved in DMSO or DMF before further dilution in aqueous buffers

Medium length spacer arm (total length added to target) is 32.6 angstroms; contains cyclohexane ring, which stabilizes adjacent maleimide