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IODOACETYL LC BIOTIN

(Biotinylation Reagent)


B107   N-Iodoacetyl-N-biotinylhexylenediamine
  f.w.: 510.4 daltons

Iodoacetyl LC Biotin is a medium length, haloacetyl activated, sulfhydryl reactive biotinylation reagent that forms stable, irreversible thioether bonds at alkaline pH.



Quantity Price  
50mgm $94.50
100mgm $178.50
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Spacer arm 27.1 angstroms. Biotinylation reagent. Reacts specifically with thiol groups at pH 7.5-8.5 to form stable thiol-ether bond. Reagent is not water soluble so a small amount of DMSO or DMF should be added to the ligand containing buffer. Soluble in DMSO and DMF.

  1. Sutoh, K., Yamamoto, K. and Wakabayashi, T. (1984). J. Mol. Biol. 178, 323-339. 
  2. Yamamoto, K., Sekine, T. and Sutoh, K. (1984). FEBS 176, 75-78.
  • Iodoacetyl LC hydrazide biotinylates antibodies or other proteins for use in protein methods
  • It is membrane permeable and can be used to label inside cells (intracellular)
  • Thiol reactive - Iodoacetyl LC biotin reacts with sulfhydryls (-SH), such as the side-chain of cysteine
  • Iodoacetyl activated It can perform reactions in the dark at pH 7.5 to 8.5 in Tris or borate buffer
  • Iodoacetyl biotin is irreversible and forms permanent thioether bonds; spacer arm cannot be cleaved
  • Must be dissolved in DMSO or DMF before further dilution in aqueous buffers
  • Medium length spacer arm (27.1 angstroms). Iodoacetyl LC biotin contains a hexylenediamine extension.