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DTNB Ellman's Reagent

(Chromogenic Substrate)

CS106   5,5'-Dithio-bis ( 2-nitrobenzoic acid )
[ 69-78-3 ]   f.w.: 396.4 daltons

DTNB, Ellman's reagent, is a versatile, water soluble compound for quantifying free sulfhydryl groups in solution. It reacts with a free sulfhydryl group to yield a mixed disulfide and 2-nitro-5-thiobenzoic acid (NTB), a measurable yellow colored product at 412 nanometers.
Ellman's reagent is very useful as a free sulfhydryl assay reagent due to its high specificity for -SH groups at neutral pH, high molar extinction coefficient and short reaction time. DTNB is a yellow crystalline powder and used for the total determination of thiol groups in biological samples.

Quantity Price  
2gm $39.00
5gm $92.00
10gm $175.00

1.Ellman, G.L. (1959) Tissue sulfhydryl groups. Arch Biochem Biophys 82:70-7.
2.Riddles, P.W., et al. (1983) Reassessment of Ellman’s reagent. Meth Enzymol 91:49-60.
3.Riddles, P.W., et al. (1979) Ellman’s reagent: 5, 5'-dithiobis(2-nitrobenzoic acid) - a reexamination. Anal Biochem 94:75-81.
4.Kuwata, K., et al. (1982) Liquid chromatographic determination of alkylthiols via derivatization with 5, 5’- dithiobis(2-nitrobenzoic acid). Anal Chem 54:1082-7.
5.Masamune, S, et al. (1989) Bio-Claisen condensation catalyzed by thiolase from Zoogloea ramigera. Active site cysteine residues. Chemtracts-Organic Chem 2:247-51.
6.Tsukamoto, Y. and Wakil, S.J. (1988) Isolation and mapping of the beta-hydroxyacyl dehydratase activity of chicken liver fatty acid synthase. J Biol Chem 263:16225-9.
7.Alvear, M., et al. (1989) Fractionation of chicken liver mevalonate 5-diphosphate decarboxylase by sulfhydryl-directed reagents: evidence of a functional dithiol. Biochem Biophys Acta 994:7-11.
• Used for the quantificaion of sulfhydryls in biological samples
• Water soluble
• Store at 2-8C