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BS3 BSSS

(Protein Crosslinker homobifunctional)


CL113   Bis(sulfosuccinimidyl)suberate
[ 82436-77-9 ]   f.w.: 572.4 daltons

BS3 (Sulfo DSS) bis(sulfosuccinimidyl)suberate, is an amine to amine crosslinker that is homobifunctional, water soluble, non cleavable and membrane impermeable.



Quantity Price  
50mgm $63.90
100mgm $120.70
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Spacer arm 11.4 angstroms. Membrane impermiable crosslinker. Non-cleavable amine reactive water soluble crosslinker used for radiolabeling ligands to cell receptor surfaces. Purity >90% by HPLC.

  1. Dihazi, G.H. and Sinz, A. (2003). Rapid Commun. Mass Spectrom. 17, 2005-2014. 
  2. Koller, D., et al. (2004) Selective Inactivation of Adrenomedullin over Calcitonin Gene-related Peptide Receptor Function by the Deletion of Amino Acids 14-20 of the Mouse Calcitonin-like Receptor. J. Biol. Chem. 279, 20387-20391. 
  3. Law, B.K., et al. (2002) Rapamycin Potentiates Transforming Growth Factor ß-Induced Growth Arrest in Nontransformed, Oncogene-Transformed, and Human Cancer Cells. Mol. Cell. Biol. 22, 8184-8198. 
  4. Mattson, G., et al. (1993). A practical approach to crosslinking. Molecular Biology Reports 17, 167-183. 
  5. Muller, D.R., et al. (2001). Anal. Chem. 73, 1927-1934. 
  6. Partis, M.D., et al. (1983). Crosslinking of proteins by omega-maleimido alkanoyl N-hydroxysuccinimide esters. J. Protein. Chem. 2, 263-277. 
  7. Sinz, A. (2003). J. Mass Spectrom. 38, 1225-1237. 
  8. Schutz, D.M., et al. (2004). Biochemistry 43, 4703-4715.

•Amine reactive Sulfo NHS ester reacts rapidly with any primary amine containing molecule

•BS3 is water soluble, so crosslinking can be done in physiologic solutions

•Water-soluble crosslinking reagent is ideal for cell surface crosslinking

•High-purity, crystalline BS3 can be used to create high purity crosslinked conjugates