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DSP

(Protein Crosslinker homobifunctional)


CL102   Dithiobis(succinimidylpropionate)
[ 57757-57-0 ]   f.w.: 404.4 daltons

DSP (Lomant\'s Reagent) is an amine reactive crosslinker having NHS ester reactive ends and a cleavable disulfide bond in its spacer arm.



Quantity Price  
1gm $144.00
5gm $680.00
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Spacer arm 12.0 angstroms. Cleaveable homobifunctional reagent. A thiol cleaveable crosslinking reagent. To reduce the disulfide bond use 50mmoles DTT, 100mmoles 2-mercaptoethanol or 1% sodium borohydride.

  1. Adir, N. and Ohad, I. (1986). Biochim. Biophys. Acta 850(2), 264-274. 
  2. Appenzeller-Herzog, C., et al. (2005) Carbohydrate- and Conformation-dependent Cargo Capture for ER-Exit Mol. Biol. Cell. 16, 1258-1267. 
  3. Joshi, S. and Burrows, R. (1990). AT synthase complex from bovine heart mitochondria. J. Biol. Chem. 265, 14518-14525. 
  4. Leduc, M., et al. (1989). Microbiol. Lett. 51(1), 11-14. 
  5. Mattson, G., et al. (1993). A practical approach to crosslinking. Molecular Biology Reports 17, 167-183. 
  6. Partis, M.D., et al. (1983). Crosslinking of proteins by omega-maleimido alkanoyl N-hydroxysuccinimide esters. J. Protein. Chem. 2, 263-277. 
  7. Xiang, C.C., et al. (2004) Using DSP, a reversible crosslinker, to fix tissue sections for immunostaining, microdissection and expression profiling. Nucleic Acids Res.32, e185.
  • Amine reactive NHS ester reacts rapidly with any primary amine containing molecule
  • Disulfide bond in the spacer arm is readily cleaved by 10-50 mM DTT or TCEP at pH 8.5
  • Spacer arm is also cleaved with 5% ?-mercaptoethanol in SDS-PAGE sample loading buffer at 100°C for 5 minutes
  • Cleavable crosslinker allows separation of crosslinked products
  • DSP crosslinker is membrane permeable, so intracellular crosslinking can be done