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BIOTIN LC HYDRAZIDE

(Biotinylation Reagent)


B109   Biotinamidocaproylhydrazide
[ 109276-34-8 ]   f.w.: 371.5 daltons

Hydrazide LC biotin is a medium length, hydrazide activated biotinylation reagent for labeling glycoproteins and other carbohydrate containing compounds having oxidizable sugars or aldehydes.



Quantity Price  
50mgm $76.50
100mgm $144.50
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Spacer arm 24.7 angstroms. Carbohydrate reactive biotinylation reagent. Biotinylates oxidized sugars and glycoproteins with a six carbon spacer between biotin and the ligand alleviating steric hindrance. Purity > 95% by TLC. Soluble in methanol and water.

  1. O\'Shannessey, D.J., et al. (1984) A novel procedure for labeling immunoglobulins by conjugation to oligosaccharide moieties. Immunol. Lett. 8, 273-277. 
  2. Kähne, T. and Ansorge, S. (1994) Non-radioactive labeling and immunoprecipitation analysis of leukocyte surface proteins using different methods of protein biotinylation. J. Immunol. Methods 168, 209-218. 
  3. Bayer, E.A., et al. (1988) Biocytin hydrazide-a selective label for sialic acids, galactose, and other sugars in glycoconjugates using avidin-biotin technology. Anal. Biochem. 170, 271-281.O’Shannessey, D.J. and Quarles, R.H. (1987) Labeling of the oligosaccharide moieties of immunoglobulins. J. Immunol. Meth. 99, 153-161. 
  4. O\'Shannessy, D. J. and Quarles R.H. (1985) Specific conjugation reactions of the oligosaccharide moieties of immunoglobulins. J. Appl. Biochem. 7, 347-355. 
  5. O\'Shannessey, D.J., et al. (1987) Quantitation of glycoproteins on electroblots using the biotin-streptavidin complex. Anal. Biochem. 163, 204-209. 
  6. Reisfield, A., et al. (1987) Nonradioactive hybridization probes prepared by the reaction of biotin hydrazide with DNA. Biochem. Biophys. Res. Com. 142, 519-526. 
  7. Rosenberg , M.B., et al. (1986) Receptor binding activities of biotinylated derivatives of ß-nerve growth factor. J. Neurochem. 46, 641-648. 
  8. Scott, M.G., et al. (2000) Cationic Antimicrobial Peptides Block the Binding of Lipopolysaccharide (LPS) to LPS Binding Protein. J. Immunol. 164, 549-553. 
  9. Wade, D.P., et al. (1985) Detection of the low density-lipoprotein receptor with biotin-low density lipoprotein. Biochem. J. 229, 785-790.

•Biotin LC hydrazide biotinylates glycosylated proteins at sialic acid residues for detection or purification using streptavidin probes or resins

•Biotinylate and isolate cell surface glycoproteins

•Aldehyde reactive reacts with aldehydes formed by periodate oxidation of sugar groups

•Hydrazide activated perform reactions at pH 4 to 6 in buffers such as sodium acetate

•Biotin LC hydrazide is irreversible and forms semi-permanent hydrazone bonds; spacer arm cannot be cleaved

•Solubility-usually dissolved in DMSO before further dilution in aqueous buffers

•Spacer arm 24.7 angstroms