SULFO SMCC

(Protein Crosslinker heterobifunctional)

CL207

Sulfosuccinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate

CAS#: [ 92921-24-9 ]

Formula Weight: f.w.: 436.4 daltons

Sulfo SMCC is a very useful,water soluble, amine to sulfhydryl reactive crosslinker that contains NHS ester and maleimide reactive groups at opposite ends of a medium length cyclohexane spacer arm (8.3 angstroms).

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Pricing & Ordering

Quantity Price Action
50mgm + $143.00
100mgm + $270.00
Product Documents
Certificate of Analysis


  • Ideal crosslinker for enzyme labeling of antibodies, both enzyme activity and antibody specificity can be preserved
  • Create specific bioconjugates via one- or two-step crosslinking reactions
  • Create sulfhydryl-reactive, maleimide-activated carrier proteins for coupling haptens
    

  1. Muller L. et al. (2010) Evolutionary gain of function for the ER membrane protein Sec62 from yeast to humans. Mol. Biol. Cell. 21, 691-703. 
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  2. Dickgreber N. et al. (2009) Targeting antigen to MHC class II molecules promotes efficient cross-presentation and enhances immunotherapy. J. Immunol. 182, 1260-9. 
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  3. Kersemans V. et al. (2008) Drug-resistant aml cells and primary aml specimens are killed by 111In-anti-CD33 monoclonal antibodies modified with nuclear localizing peptide sequences. J. Nucl. Med. 49, 1546-54. 
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  4. Duval M. et al. (2008) A bispecific antibody composed of a nonneutralizing antibody to the gp41 immunodominant region and an anti-CD89 antibody directs broad human immunodeficiency virus destruction by neutrophils. J. Virol. 82, 4671-4. 
    5. 

  5. Costantini D. L. et al. (2008) Trastuzumab-resistant breast cancer cells remain sensitive to the auger electron-emitting radiotherapeutic agent 111In-NLS-Trastuzumab and are radiosensitized by methotrexate. J. Nucl. Med. 49, 1498-505. 
    6. 

  6. Brinkmann M. M. et al. (2007) The interaction between the ER membrane protein UNC93b and TLR3, 7, and 9 is crucial for TLR signaling. J. Cell Biol. 177, 265-75. 
    7. 

  7. Kehoe J. W. et al. (2006) Using phage display to select antibodies recognizing post-translational modifications independently of sequence context. Mol. Cell. Proteomics. 5, 2350-63.
    8. 

    

  • Amine reactive Sulfo NHS ester crosslinks rapidly with primary amine-containing molecule 
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  • Sulfhydryl-reactive maleimide reacts with cysteine residues to yield specific conjugates 
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  • Sulfo SMCC is water soluble, so crosslinking can be done in physiologic solutions 
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  • High purity, crystalline Sulfo-SMCC can be used to create high-purity maleimide-activated derivatives 
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  • Cyclohexane bridge confers added stability to the maleimide group making Sulfo SMCC the ideal crosslinking agent for maleimide activation of proteins.
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