Protein Modification Reagents TCEP
Economically equivalent, odor free reductions at DTT prices available in large quantities with very high purity and stability. In addition Soltec Ventures offers a TCEP stable solution. The solution is 0.5M and stable at room temperature years under inert atmosphere.
| Unit | Price |
| 1gm | $22.00 |
| 2gm | $30.00 |
| 5gm | $52.00 |
| 10gm | $95.00 |
| 25gm | $230.00 |
M115 Tris(2-carboxyethyl)phosphine hydrochloride, TCEP
f.w.: 286.6daltons [ 51805-45-9 ]
Stability!
- Odorless and non-volatile
- Solid is stable in air for several months
- Dilute TCEP solutions in air show no appreciable oxidation up to at least 72 hours pH<7.66
Solubility!
310g/l in water
Reactivity!
- TCEP reduces organic disulfides to thiols rapidly and quantitatively in water
- More complete reductions than 1,4-dithiothreitol ( DTT ), Cleland's reagent
- Even very stable alkyl disulfides are completely reduced at room temperature and pH 5 in less than 5 min.
- Dilute (1mM) TCEP solutions react rapidly at room temperature
- The strength of the phosphorus-oxygen bond makes the reaction irreversible
- Kinetics rather than thermodynamics controls the reduction
Selectivity!
- TCEP is selective for disulfides
- TCEP does not react with other functional groups on proteins
- Unreactive towards many common alkylating reagents so reductions have been carried out simultaneously with alkylations
TCEP References:
1. Kirkley, T.L., Anal. Biochem, 180, 231-236 (1989).
2. Andrews, P. C., Dixon, J.E., Anal. Biochem, 161, 524-528 (1987).
3. Schonberg, A., Chem Ber., 163-164 (1935).
4. Rauhut, M., et. al., JACS, 81, 1103-1107 (1959).
5. Chen, C.S., Fujimoto, Y., Girdoukas, G.Sih, C.J., JACS, 109, 6825-6309 (1987).
6. Houk, J., Whitesides, G.M., JACS, 109, 6825-6836 (1987).
7. Grayson, M., Farley, C.E., Chimie Organique du Phophore, Collogues Int'l du Centre Nat'l de la Recherche Scientifique, No. 182 CNRS, Paris, 275-284 (1970).
8. Overman, L.E., O'Conner, E.M., JACS, 98, 771-775 (1977).
9. Rosenfield, R.E., Parthasarthy, R., Dunitis, J.D., JACS, 99, 4860-4862 (1977).