Biotinylation Reagents Sulfo NHS SS Biotin
the disulfide bond in the spacer arm is cleavable using reducing agents such as TCEP and DTT
B105 Sulfosuccinimidyl-2-(biotinamido)ethyl-1,3-dithiopropionate
f.w.:606.7daltons
Spacer arm 24.3 angstroms
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Sulfo NHS SS biotin biotinylates, amino acids, peptides or proteins under mild conditions
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Ideal for reversible biotinylation
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Cleavable water soluble analog to NHC LC Biotin
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Soluble in water
unit price 50mgm $85.50 100mgm $161.50 Download Certificate of Analysis Sulfo NHS SS Biotin has a spacer arm 24.3 angstroms long. It is a cleavable biotinylation reagent. Sulfo NHS SS Biotin is a water soluble biotinylation reagent that is reversible. It is useful for extracting non covalently associated proteins from complex physiological mixtures under mild isolation conditions. Soluble in water. -
Forms stable amide bonds
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Extended spacer arm reduces steric hindrance
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Each product is accompanied with a certificate of analysis.
Sulfo NHS SS Biotin References:
- Shimkus, M., et al, Proc. Natl. Acad. Sci., 82, 2593-2597 (1985).
- Duhamel, R.C., Johnson, D.A., J. Histochem. Cytochem., 33, 711-714 (1985).
- Gottardi, C.J., et al, Am. J. Physiol., 268(2), F285-F295 (1995).
- Soukup, G.A., et al, Bioconjugate Chem., 6, 135-138 (1995)
- Daniels, G.M., Amara,S.G., Methods Enzymol., 296, 307-318 (1998).
- Huh, K.H., Wenthold, R.J., J. Biol. Chem., 274, 151-157 (1999).
- Trotti, D., et al, J. Biol. Chem., 276, 576-582 (2001).