NDSB 201 protein renaturation and refolding reagent available

Non Detergent Sulfobetaines NDSB 201

SB201 3-(1-Pyridino)-1-propane sulfonate, 99+%

[ 15471-17-7 ] f.w.:201.2daltons

prevents protein aggregation
facilitates the renaturation of chemically and thermally denaturated proteins
zwitterionic over a wide pH range
easily removed by dialysis

unit	price
5gm	$19.00
25gm	$75.00
100gm	$240.00
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We manufacture these products so bulk quotations are always available with substantial savings.

Each product is accompanied with a certificate of analysis.

NDSB 201 references:

1. Vuillard, L., et al, Anal. Biochem, 230, 290 (1995)
2. Vuillard, L., et al, J. Biochem., 305, 337 (1995)
3. Vuillard, L., et al, FEBS Lett, 353, 294 (1994)
4. Goldberg, M.E., et al, Folding and Design, 1, 21 (1996)
5. FEBS Lett 1994 Oct 24;353(3):294-6
6. Biochem J 1995 Jan 1;305 ( Pt 1):337-43
7. Electrophoresis 1995 Mar;16(3):295-7
8. Fold Des 1996;1(1):21-7
9. Eur J Biochem 1998 Aug 15;256(1):128-35
10. J.Cryst Growth (1996) vol 168 pp 150-154
11. Anal. Biochem. (1995) 230, 290

NDSB 201 is a non detergent sulfobetaine that prevents protein aggregation and facilitates the renaturation of chemically and thermally denatured proteins. Most enzymes remain active in the presence of these reagents. The applications are wide ranging in protein biochemistry useful to enhance extraction, solubilization and crystalization. The non detergent sulfobetaine reagents are zwitterionic, very soluble in water ( greater than 2.0 molar), do not alter significantly the pH or viscosity of biological buffers and can easily be removed by dialysis.

NDSBs contain a hydrophilic sulfobetaine group and a short hydrophobic group. Hence, NDSBs cannot form micelles and are not considered detergents. Despite this, NDSBs have been used successfully to increase the yields of membrane, nuclear, and cytoskeletal associated proteins.It has been suggested that the NDSBs short hydrophobic group interacts with the hydrophobic regions on proteins to prevent aggregation. An analogy for using NDSB non detergent sulfobetaines with proteins and detergents would be to look at these reagents as fabric softeners.

Examples: NDSB 201 does not interfere wilth colorimetric assays using p-nitrophenylphosphate and o-nitrophenyl beta galacotopyraniside as substrates.