Non Detergent Sulfobetaines NDSB 195
SB195 Dimethylethylammonium propane sulfonate, 99+%
f.w.:195.3daltons
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prevents protein aggregation
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facilitates the renaturation of chemically and thermally denaturated proteins
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zwitterionic over a wide pH range
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easily removed by dialysis
| unit | price |
| 5gm | $34.20 |
| 10gm | $70.00 |
| 25gm | $161.50 |
| Download***Certificate of Analysis*** |
NDSB 195 is a non detergent sulfobetaine that prevents protein
aggregation and facilitates the renaturation of chemically and
thermally denatured proteins. Most enzymes remain active in the
presence of these reagents. The applications are wide ranging in
protein biochemistry useful to enhance extraction,
solubilization and crystalization. The non detergent
sulfobetaine reagents are zwitterionic, very soluble in water (
greater than 2.0 molar), do not alter significantly the pH or
viscosity of biological buffers and can easily be removed by
dialysis.
NDSBs contain a hydrophilic sulfobetaine group and a short hydrophobic group. Hence, NDSBs cannot form micelles and are not considered detergents. Despite this, NDSBs have been used successfully to increase the yields of membrane, nuclear, and cytoskeletal associated proteins.It has been suggested that the NDSBs short hydrophobic group interacts with the hydrophobic regions on proteins to prevent aggregation. An analogy for using NDSB non detergent sulfobetaines with proteins and detergents would be to look at these reagents as fabric softeners.
Examples: At a concentration of 0.25M NDSB-195 the solubility of lysozyme almost doubles, whereas at 0.75 M NDSB-195 the solubility is nearly tripled.
NDSBs contain a hydrophilic sulfobetaine group and a short hydrophobic group. Hence, NDSBs cannot form micelles and are not considered detergents. Despite this, NDSBs have been used successfully to increase the yields of membrane, nuclear, and cytoskeletal associated proteins.It has been suggested that the NDSBs short hydrophobic group interacts with the hydrophobic regions on proteins to prevent aggregation. An analogy for using NDSB non detergent sulfobetaines with proteins and detergents would be to look at these reagents as fabric softeners.
Examples: At a concentration of 0.25M NDSB-195 the solubility of lysozyme almost doubles, whereas at 0.75 M NDSB-195 the solubility is nearly tripled.
We manufacture these products so bulk quotations are always available with substantial savings.
Each product is accompanied with a certificate of analysis.
NDSB 195 references
1. Vuillard, L., et al, Anal. Biochem, 230, 290 (1995)2. Vuillard, L., et al, J. Biochem., 305, 337 (1995)
3. Vuillard, L., et al, FEBS Lett, 353, 294 (1994)
4. Goldberg, M.E., et al, Folding and Design, 1, 21 (1996)
5. FEBS Lett 1994 Oct 24;353(3):294-6
6. Biochem J 1995 Jan 1;305 ( Pt 1):337-43
7. Electrophoresis 1995 Mar;16(3):295-7
8. Fold Des 1996;1(1):21-7
9. Eur J Biochem 1998 Aug 15;256(1):128-35
10. J.Cryst Growth (1996) vol 168 pp 150-154
11. Anal. Biochem. (1995) 230, 290