Non Detergent Sulfobetaines ( NDSB )
Soltec Ventures offers a comprehensive listing of non detergent sulfobetaines for protein research. Non detergent sulfobetaines (NDSB 's) are used for protein folding and protein renaturation.
Click on a link below to order one of our non detergent sulfobetaines (NDSB 's). Listed will be the structure, properties and references to important protein research references.
Soltec Ventures manufactures kilogram quantities of these products. Call for a bulk quotation or email at quote@soltecventures.com.
To order-
- phone: 978 922 9446
- fax: 978 922 1666
- email: sales@soltecventures.com
Click on the link below for a particular product.
NDSB 195 NDSB 201 NDSB 211 NDSB 221 NDSB 256
The NDSB 's are a group of zwitterionic compounds that can reduce aggregation and aid in refolding proteins found in inclusion bodies and bacterial expression systems. Non detergent sulfobetaines ( NDSB ' s ) have a sulfobetaine hydrophilic group and a short hydrophobic group that cannot aggregate to form micelles, therefore NDSB 's are not considered detergents. NDSB 's increase the extraction yield ( up to 30% ) of membrane, nuclear and cyto-skeletal associated proteins. The short hydrophobic groups combined with the charge neutralization of the sulfobetaine group results in higher yields of membrane proteins. NDSB 's have been used in refolding and renaturation of chemically and thermally denatured proteins.
The non detergent sulfobetaines are zwitterionic over a wide pH range, easily removed by dialysis and do not absorb significantly in the near UV range.
NDSB Non detergent sulfobetaine references:
1. Vuillard, L., et al, Anal. Biochem, 230, 290 (1995)
2. Vuillard, L., et al, J. Biochem., 305, 337 (1995)
3. Vuillard, L., et al, FEBS Lett, 353, 294 (1994)
4. Goldberg, M.E., et al, Folding and Design, 1, 21 (1996)
5. Blisnick, T. et al, Eur. J. Biochem., 252, 537 (1998)
6. Ochem, A.E., et al, J. Biol. Chem., 272, 29919 (1997)
7. Benetti, P.H., et al, Protein Expr. Purif., 13, 283 (1998)
8. Vuillard, L., et al, Eur. J. Biochem., 256, 128 (1998)
9. Righetti, P.G., et al, J. Capillary Electrophor., 4, 47 (1997)