GC Diagnostic Reagents HFBI
Acylation derivatization reagent
Perfluoroacylimidazoles offer effective acylation of hydroxyl groups, primary and secondary amines.
GC108 Heptafluorobutryl imidazole, 99+%
[ 32477-35-3 ] f.w.:264.1daltons
b.p. 161°; d. 1.490
·
- Offer considerable advantages over the anhydrides for the preparation of perfluoroacyl derivatives; the reactions are smooth, quantitative and produce no acid byproducts that must be removed from the system before injection.
- Principal byproduct is imidazole (relatively inert)
- React with hydroxyl groups, primary and secondary amines, and quantitatively acylate indole alkylamines
- Tryptamine and metabolites present in spinal fluid have been analyzed by ECD using HFBI
- Used in bifunctional derivatization schemes and in exchange reactions where TMS derivatives are converted to HFB derivatives
- Hydroxyl groups of catecholamines are derivatized with TMSI, followed by conversion of the amines to acylamides with HFBI
The products are packaged in hypovials with septum inserts for ease in sampling and use.
We manufacture these products so bulk quotations are always available with substantial savings.
Each product is accompanied with a certificate of analysis.
References:
- Bennington, F., et.al. (1975). Identification and separation of indolealkylamines by gas-liquid chromatographic analysis of their heptafluorobutyryl derivatives.J. Chromatogr. 106:435-9.
- Seeley, S.D. and Powell, L.E. (1974). Gas chromatography and detection of microquantities of gibberellins and indoleacetic acid as their fluorinated derivatives. Anal. Biochem. 58:39-46.
- Horning, M.G., et.al. (1968). The GLC separation of hydroxyl-substituted amines of biological importance including the catecholamines. Preparation of derivatives for electron capture detection. Anal. Lett. 1(5):311-21.
| unit | price |
| 5gm | $41.00 |
| 10gm | $65.00 |
| 25gm | $99.00 |
