Biotin HPDP has a spacer arm 29.2 angstroms long. It is a
sulfhydryl reactive biotinylating reagent. Biotinylation occurs
between pH 6-9., the pyridyl dithiol reacts with sulfhydryl
groups to form a stable sulfhydryl bond. The pyridine-2-thione
leaving group can be measured at 343nm to determine the degree
of biotinylation. Purity >94% by HPLC.
Biotinylation Reagents Biotin HPDP
A reversible biotinylation reagent, the disulfide bond in the spacer arm is cleavable using reducing agents such as TCEP and DTT
B106 N-[6-(Biotinamido)hexyl]-3'-(2'-pyridyldithio)propionamide
f.w.:539.8daltons
Spacer arm 29.2 angstroms
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Biotinylates, amino acids, peptides or proteins under mild conditions
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Ideal for reversible biotinylation
unit price 50mgm $130.50 100mgm $246.50 Download Certificate of Analysis -
Pyridyldithio group reacts with sulfhydryls to form a stable disulfide bond
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Soluble in DMSO and DMF
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Extended spacer arm reduces steric hindrance
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Biotinylation occurs between pH 6-9
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Each product is accompanied with a certificate of analysis.
Biotin HPDP References:
- Ghebrihiwet, B., et al, J. Immunol. Methods, 110, 251-260 (1988).
- Ishmael, F.T., et al, J. Biol. Chem., 276, 25236-25242 (2001).
- Slatin, S.L., et al, Proc. Natl. Acad. Sci. USA, 99, 1286-1291 (2002).