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BCA Protein Assay

 

• BCA Cu⁺¹ color complex is stable
• Little susceptibility to detergents ( see compatibility chart )
• BCA is applicable over a broad range of protein concentrations

  stock #	unit	price
  BCA 250	250ml solution	$30.00
  BCA 500	500ml solution	$55.00
  BCA 1000	1000ml solution	$80.00
  BCA 3750	3.75liter solution	$300.00

  stock #	unit	price
  BCA 2	2gm	$30.00 Data not available (103) Return to Previous Page

  ' "> BCA 5 5gm 65.00

  Data not available (103)

  Return to Previous Page' ">

  BCA 25 25gm 240.00

  stock #	unit	price
  BCA 250	250 determinations kit	$42.00
  BCA 500	500 determinations kit	$75.00
  BCA 1000	1000 determinations kit	$95.00
  BCA 3750	1 gallon kit	$340.00

  Protein determination is one of the most common operations performed in biochemical research. The principle of the bicinchoninic acid ( BCA ) assay is similar to the Lowry procedure, in that both rely on the formation of a Cu⁺² protein complex under alkaline conditions, followed by reduction of the Cu⁺² to Cu⁺¹.

   The amount of reduction is proportional to the protein present. It has been shown that cysteine, trytophan, tyrosine and the peptide bond are able to reduce Cu⁺² to Cu⁺¹. BCA - bicinchoninic acid forms a purple blue complex with Cu⁺¹ in alkaline solutions. This provides a basis to monitor the reduction of alkaline Cu+2 by proteins and thus determine the protein concentration in a biochemical sample.

  Bicinchoninic acid is a highly chromogenic reagent when complexed with Cu⁺¹, forming a purple blue complex with an absorbance maximum  at 562 nanometers. The absorbance is directly proportional to the protein concentration.

BCA Solutions Available

 

 

 

 

 

 

 

 

 

 

 

BCA Bicinchonnic sodium salt

 

 

 

 

 

 

 

 

 

BCA Kits Available

 

 

 

 

 

 

 

 

 

 

 

BCA References:

    1. Smith, P.K., et al, Anal. Biochem., Measurement of Protein Using Bicinchoninic Acid, 150, 76-85 (1985)

   

 

Compatability with Other Reagents