BCA Protein Assay
- BCA Cu+1 color complex is stable
- Little susceptibility to detergents ( see compatibility chart )
- BCA is applicable over a broad range of protein
concentrations
stock # unit price BCA 250 250ml solution $30.00 BCA 500 500ml solution $55.00 BCA 1000 1000ml solution $80.00 BCA 3750 3.75liter solution $300.00 stock # unit price BCA 2 2gm $30.00 BCA 5 5gm $65.00 BCA 25 25gm $240.00 stock # unit price BCA 250 250 determinations kit $42.00 BCA 500 500 determinations kit $75.00 BCA 1000 1000 determinations kit $95.00 BCA 3750 1 gallon kit $340.00 Protein determination is one of the most common operations performed in biochemical research. The principle of the bicinchoninic acid ( BCA ) assay is similar to the Lowry procedure, in that both rely on the formation of a Cu+2 protein complex under alkaline conditions, followed by reduction of the Cu+2 to Cu+1.
The amount of reduction is proportional to the protein present. It has been shown that cysteine, trytophan, tyrosine and the peptide bond are able to reduce Cu+2 to Cu+1. BCA - bicinchoninic acid forms a purple blue complex with Cu+1 in alkaline solutions. This provides a basis to monitor the reduction of alkaline Cu+2 by proteins and thus determine the protein concentration in a biochemical sample.
Bicinchoninic acid is a highly chromogenic reagent when complexed with Cu+1, forming a purple blue complex with an absorbance maximum at 562 nanometers. The absorbance is directly proportional to the protein concentration.
BCA Solutions Available
BCA Bicinchoninic sodium salt
| Substance | Amount |
| CHAPSO | 1.0% |
| CHAPS | 1.0% |
| Nonident P-40 | 1.0% |
| DTT | 1mM |
| SDS | 1.0% |
| Triton x-100 | 1.0% |
| Triton x-114 | 1.0% |
| Tween 20 | 1.0% |
| Tween 80 | 1.0% |
| Octyl beta glucocide | 1.0% |
| Ammonium sulfate | 1.5M |
| Glycine | 0.1M |
| HEPES | 50mM |
| Imidazole | 25mM |
| MES | 0.1M |
| Sodium acetate | 0.2M |
| Sodium bicarbonate | 0.1M |
| Sodium chloride | 1.0M |
| Sodium phosphate | 0.1M |
| Tris | 0.25M |
| Dithioethyritol | 1mM |
| 2-Mercaptoethanol | 0.01% |
| EDTA | 10mM |
| Guanidine HCl | 4.0M |
| Methanol | 10% |
| Urea | 3.0M |
BCA Kits Available
BCA References:
1. Smith, P.K., et al, Anal. Biochem., Measurement of Protein Using Bicinchoninic Acid, 150, 76-85 (1985)
Compatability with Other Reagents