Alkylphosphine oxides ( APO ) are a family of non ionic
detergents with a wide range of versatile uses. The detergents
are pure crystalline solids, soluble in water and the solutions
are stable over long periods. Ideally the alkyl phosphine
detergents have low CMC values, are UV transparent and P31 NMR
detectable. Alkyl phosphine oxide detergents have been used for
purification of high purity DNA without the need to use resins
or phenol/chloroform. APO 10 has been used in the two phase
separation and purification of proteins.
CMC (H2O) ~ 4.66 mM (0.10%)
Aggregation Number (H2O) ~ 31
Purity 99% pure by HPLC analysis.
pH (1% solution) 5-8
Solubility in water at 0-5°C 20%
Conductance (10% solution) < 100 µS
Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10
Absorbance of a 1% detergent solution:
340 nm < 0.05
280 nm < 0.08
260 nm < 0.1
CMC (H2O) ~ 4.66 mM (0.10%)
Aggregation Number (H2O) ~ 31
Purity 99% pure by HPLC analysis.
pH (1% solution) 5-8
Solubility in water at 0-5°C 20%
Conductance (10% solution) < 100 µS
Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10
Absorbance of a 1% detergent solution:
340 nm < 0.05
280 nm < 0.08
260 nm < 0.1
Purified Detergents APO 10
APO 10 is a novel non ionic alkyl phosphine oxide detergent.
S210 Dimethyldecylphosphine oxide, 98%
[ 2190-95-6 ] f.w.:218.3daltons
| unit | price |
| 1gm | $50.00 |
| 5gm | $240.00 |
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Novel non ionic detergent useful in two phase separation and purification of proteins
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APO 10 gives excellent yields of high quality DNA
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Purification of plasmid DNA using APO 10 does not require the use of chloroform / phenol or resins
We manufacture these products so bulk quotations are always available with substantial savings.
Each product is accompanied with a certificate of analysis.
APO 10 references:
- Altschuler, M., et al, Biotechniques, 17, 434, (1994).
- Hermann, K.,W., Brushmiller, J.G., Courchene, W.L., Micellar Properties of Dimethylalkylphosphine Oxide Solutions, 70(9), 2909, (1966).
- Kresheck, G.C., and Huang J., Chem Phys. Lipids, 76, 193, (1995 ).