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TCEP Solution(Protein Modifier) M116 Tris(2-carboxyethyl)phosphine (neutral solution) 500mM in water (0.5M)[ 51805-45-9 ] f.w.: 250.1 daltons |
TCEP solution is a potent versatile odorless thiol free reducing agent with many applications in protein chemistry and proteomic research centered in the quantitative ease of reducing disulfide bonds. The versatile compound is readily soluble and very stable in aqueous solutions. In fact TCEP solution is stable in aqueous, acidic and basic solutions. The neutral solution has a shelf life of one year. TCEP solution reduces disulfide bonds as effectively as dithiothreitol ( DTT ), but unlike dithiothreitol ( DTT ) and other thiol reducing agents, it does not need to be removed prior to certain sulfhydryl reactive cross linking reactions. These are but a few of the reasons TCEP solution is a superior reagent than dithiothreitol ( DTT ) for use in reducing disulfide bonds in protein chemistry and proteomic research.
| Quantity | Price | |
|---|---|---|
| 1ml + | $39.00 |  |
| 2ml + | $74.00 | |
| 5ml + | $167.00 | |
| 10ml + | $210.00 | |
| 25ml + | $706.00 | |
| Download***Certificate of Analysis*** | ||
Product is a superior alternative to DTT. The product is odorless, non-volatile and stable in air. Dilute solutions in air show no appreciable oxidation. Reduces organic disulfides to thiols rapidly and quantitatively in water. The reaction is reversible. Does not react with other functional groups on proteins.
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- Odorless and non-volatile
- Solutin is stable at room temperature for one year is stable in air for several months
- Dilute TCEP solutions in air show no appreciable oxidation up to at least 72 hours pH<7.66
- Solubility in water 310gm/liter
- TCEP reduces organic disulfides to thiols rapidly and quantitatively in water
- More complete reductions than 1,4-dithiothreitol ( DTT ), Clelands reagent
- Even very stable alkyl disulfides are completely reduced at room temperature and pH 5 in less than 5 min.
- Dilute (1mM) TCEP solutions react rapidly at room temperature
- The strength of the phosphorus-oxygen bond makes the reaction irreversible
- Kinetics rather than thermodynamics controls the reduction Selectivity!
- TCEP is selective for disulfides
- TCEP does not react with other functional groups on proteins
- Unreactive towards many common alkylating reagents so reductions have been carried out simultaneously with alkylations